Product overview:

Carrier-free (BSA/glycerol-free) RASSF1 mouse monoclonal antibody, clone LBI8E4

Field of research:

Bladder cancer, Non-small cell lung cancer, Pathways in cancer

Summary:

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Alternative names:

123F2; NORE2A; RASSF1A; RDA32; REH3P21

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

RASSF1

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-341 of human RASSF1(NP_009113) produced in E.coli.

Clone:

LBI8E4

Conjugation:

Unconjugated

Gene name:

Homo sapiens Ras association domain family member 1 (RASSF1), transcript variant A, mRNA.

Symbol:

RASSF1

Pathway:

Bladder cancer, Non-small cell lung cancer, Pathways in cancer

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com