Product overview:

Carrier-free (BSA/glycerol-free) ACLY mouse monoclonal antibody, clone LBI3G8

Field of research:

Citrate cycle (TCA cycle), Metabolic pathways

Summary:

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternative names:

ACL; ATPCL; CLATP

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

ACLY

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human ACLY (NP_001087) produced in HEK293T cell.

Clone:

LBI3G8

Conjugation:

Unconjugated

Gene name:

Homo sapiens ATP citrate lyase (ACLY), transcript variant 1, mRNA.

Symbol:

ACLY

Pathway:

Citrate cycle (TCA cycle), Metabolic pathways

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:50, IF 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com