Product overview:

Carrier-free (BSA/glycerol-free) FH mouse monoclonal antibody, clone LBI9G4

Field of research:

Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma

Summary:

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]

Alternative names:

FMRD; HLRCC; LRCC; MCL; MCUL1

Species reactivity:

Human, Mouse, Rat, Dog

Host:

Mouse

Target:

FH

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.

Clone:

LBI9G4

Conjugation:

Unconjugated

Gene name:

Homo sapiens fumarate hydratase (FH), mRNA; nuclear gene for mitochondrial product.

Symbol:

FH

Pathway:

Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com