Product overview:

Carrier-free (BSA/glycerol-free) DHFR mouse monoclonal antibody, clone LBI3A11

Field of research:

Folate biosynthesis, Metabolic pathways, One carbon pool by folate

Summary:

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Alternative names:

DHFRP1; DYR

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

DHFR

Isotype:

IgG2b

Immunogen:

Full-length protein expressed in 293T cell transfected with human DHFR expression vector

Clone:

LBI3A11

Conjugation:

Unconjugated

Gene name:

Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA.

Symbol:

DHFR

Pathway:

Folate biosynthesis, Metabolic pathways, One carbon pool by folate

Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB: 1:200 - 1:1000 IHC 1:50

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com