Background:

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament(F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin(124 kDa), is muscle-specific.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

CMD1W, Metavinculin, MVCL, VCL, Vinculin

Specificity:

Human, Rat, Mouse

Host:

Mouse

Isotype:

IgG1

Immunogen:

vinculin

Purification:

Protein A+G purification

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

124 kDa

Form:

liquid

Applications:

ELISA, FC, IF, IHC, WB

Recommended dilution:

WB: 1:2000-1:20000; IHC: 1:50-1:500; IF: 1:50-1:500

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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