Background:

DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

DNA annealing helicase and endonuclease ZRANB3,Annealing helicase 2,AH2

Specificity:

Human

Host:

Rabbit

Isotype:

IgG

Immunogen:

zinc finger, RAN-binding domain containing 3

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

123 kDa, observed 150 kDa and 50 kDa

Form:

liquid

Applications:

ELISA, WB

Recommended dilution:

WB: 1:200-1:2000; IF: 1:20-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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