Background:

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short(less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay(PubMed:25480299). Involved in microRNA(miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7(pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem(ES) cell pluripotency(By similarity). Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression(By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

KIAA0191, PAPD3, Terminal uridylyltransferase 4, TUT4, TUTase 4, ZCCHC11

Specificity:

Human

Host:

Rabbit

Isotype:

IgG

Immunogen:

zinc finger, CCHC domain containing 11

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

185 kDa

Form:

liquid

Applications:

ELISA, WB, IHC, IF

Recommended dilution:

WB: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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