Background:

Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element(ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72(DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1(HIV-1), moloney and murine leukemia virus(MoMLV) and xenotropic MuLV-related virus(XMRV), filoviridae: ebola virus(EBOV) and marburg virus(MARV), togaviridae: sindbis virus(SINV) and Ross river virus(RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of DDX58/RIG-I signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes(ISGs).

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

DKFZp686F2052, DKFZp686H1869, DKFZp686O19171, FLB6421, FLJ13288, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

Specificity:

Human, Mouse, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

zinc finger CCCH-type, antiviral 1

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

101 kDa

Form:

liquid

Applications:

ELISA, WB, IHC

Recommended dilution:

WB: 1:1000-1:10000; IHC: 1:20-1:200; IP: 1:1000-1:4000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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