Background:

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex(PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets(PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells(By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells(By similarity). Involved in the homotypic fusion of early and late endosomes(By similarity).

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

EDB, Endobrevin, VAMP 8, VAMP8

Specificity:

Human, Mouse, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

vesicle-associated membrane protein 8(endobrevin)

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

20 kDa, observed 20 kDa, 50 kDa

Form:

liquid

Applications:

ELISA, WB

Recommended dilution:

WB: 1:200-1:1000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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