Background:

Specifically deubiquitinates 'Lys-120' of histone H2A(H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3(H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B-and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

Deubiquitinating enzyme 16, Ubiquitin thiolesterase 16, UBP M, USP16

Specificity:

Human, Mouse, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

ubiquitin specific peptidase 16

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

112 kDa

Form:

liquid

Applications:

ELISA, WB, IHC, IP

Recommended dilution:

WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:20-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

More info:

Email: info@sobekbio.com

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