Background:

Ubiquitin-binding protein(PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin(PubMed:19328070, Ref.36). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks(DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

BRCA1 A complex subunit RAP80, RAP80, Receptor associated protein 80, RXRIP110, UIMC1, X2HRIP110

Specificity:

Human, Mouse

Host:

Rabbit

Isotype:

IgG

Immunogen:

ubiquitin interaction motif containing 1

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

164 kDa

Form:

liquid

Applications:

ELISA, WB, IF, IHC,IF

Recommended dilution:

WB: 1:200-1:1000; IP: 1：200-1：2000；IHC: 1：20-1：200; IF: 1：10-1：100

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com