Background:

Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system(UPS), autophagy and the endoplasmic reticulum-associated protein degradation(ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding(via UBA domain) to their polyubiquitin chains and by interacting(via ubiquitin-like domain) with the subunits of the proteasome(PubMed:10983987). Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome(PubMed:24215460, PubMed:18307982). Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion(PubMed:19148225, PubMed:20529957). Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits(CCPs)(PubMed:18199683).

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

CHAP1, CHAP1/DSK2, Dsk2, DSK2 homolog, hPLIC 2, HRIHFB2157, LIC 2, N4BP4, PLIC 2, PLIC2, RIHFB2157, ubiquilin 2, Ubiquilin-2, UBQLN2

Specificity:

Human, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

ubiquilin 2

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

66 kDa

Form:

liquid

Applications:

ELISA, WB, IHC, IF

Recommended dilution:

WB: 1:200-1:2000; IHC: 1:20-1:200；IF：1:20-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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