Background:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome(APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

DJ447F3.2, UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

Specificity:

Human

Host:

Mouse

Isotype:

IgG1

Immunogen:

ubiquitin-conjugating enzyme E2C

Purification:

Protein A+G purification

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

20 kDa

Form:

liquid

Applications:

ELISA, IF, IHC, WB

Recommended dilution:

WB: 1:500-1:5000; IHC: 1:20-1:200；IF: 1:10-1:100

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

More info:

Email: info@sobekbio.com

Orders:

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