Background:

Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA(ssDNA) and double-stranded DNA(dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA(ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

3 5 exonuclease TREX1, AGS1, AGS5, CRV, DKFZp434J0310, DNase III, DRN3, HERNS, TREX1

Specificity:

Human

Host:

Rabbit

Isotype:

IgG

Immunogen:

three prime repair exonuclease 1

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

132 kDa

Form:

liquid

Applications:

ELISA, WB, IP

Recommended dilution:

WB: 1:200-1:1000; IP: 1:200-1:1000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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