Background:

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

N/A

Specificity:

Human, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

replication factor C (activator 1) 4, 37kDa

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

38 kDa

Form:

liquid

Applications:

ELISA, WB, IHC, IF, IP

Recommended dilution:

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100; IP: 1:50 - 1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

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