Background:

RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site(5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

fSAP94, NET52, Protein S164, RBM25, RED120, RNA binding motif protein 25, RNA binding protein 25, RNPC7, S164

Specificity:

Human, Mouse

Host:

Rabbit

Isotype:

IgG

Immunogen:

RNA binding motif protein 25

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

110-120 kDa

Form:

liquid

Applications:

ELISA, WB, IHC

Recommended dilution:

WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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