Background:

Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65

Specificity:

Human, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

v-rel reticuloendotheliosis viral oncogene homolog A(avian)

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

65 kDa

Form:

liquid

Applications:

ELISA, IHC, IF, WB

Recommended dilution:

WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

More info:

Email: info@sobekbio.com

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