Background:

DNA glycosylase which prefers single-stranded DNA(ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA(dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin(Sp) and guanidinohydantoin(Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

N/A

Specificity:

Human, Mouse

Host:

Rabbit

Isotype:

IgG

Immunogen:

nei endonuclease VIII-like 3(E. coli)

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

62-68 kDa

Form:

liquid

Applications:

ELISA, WB

Recommended dilution:

Request info at info@sobekbio.com

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com