Background:

Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

Maltose Binding Protein, MBP, MBP Tag

Specificity:

Recombinant protein

Host:

Mouse

Isotype:

IgG2a

Immunogen:

MBP-Tag

Purification:

Protein A+G purification

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

40 kDa

Form:

liquid

Applications:

ELISA, WB, IP

Recommended dilution:

WB: 1:1000-1:8000; IP: 1:5000-1:50000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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