Background:

Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'(PubMed:11095750). Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3(PubMed:15485867). Involved in the regulation of liver cancer cells self-renewal(PubMed:25985737).

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

BHLHB31, CHF2, HERP2, HESR1, HRT1

Specificity:

Human, Mouse, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

hairy/enhancer-of-split related with YRPW motif 1

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

33 kDa

Form:

liquid

Applications:

ELISA, WB, IHC, IP

Recommended dilution:

WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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