Background:

Mouse monoclonal antibody to Alpha-actin-1

Alternative names:

ACTA; Actin alpha skeletal muscle; Alpha-actin-1

Clonality:

Monoclonal

Species reactivity:

H, M, R

Host:

Mouse

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-actin-1. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC, IP

Recommended dilution:

WB (1/5000 - 1/10000), IH (1/200 - 1/500), IP (1/100 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of Alpha-actin-1 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of Alpha-actin-1 staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

Immunoprecipitation of Alpha-actin-1 from 0.5mg mouse brain whole cell extract lysate, using anti-Alpha-actin-1 Antibody.

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com