Background:

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

RAD2

Specificity:

Human, Mouse

Host:

Rabbit

Isotype:

IgG

Immunogen:

flap structure-specific endonuclease 1

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

42 kDa

Form:

liquid

Applications:

ELISA, WB, IHC, IF, IP

Recommended dilution:

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:10 - 1:100; IP : 1:50 - 1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com