Background:

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290’s function is critical for IFT—in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

BBS14, KIAA0373, NPHP6,LCA10, MKS4, Nephrocystin 6, rd16, SLSN6, Tumor antigen se2 2

Specificity:

Human, Mouse, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

centrosomal protein 290kDa

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

290 kDa, 150-180 kDa, 80 kDa, 60 kDa

Form:

liquid

Applications:

ELISA, WB, IP, IHC, IF

Recommended dilution:

WB: 1:200-1:2000; IP: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:500

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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