Background:

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

CDC25B, CDC25HU2, M phase inducer phosphatase 2

Specificity:

Human, Mouse

Host:

Rabbit

Isotype:

IgG

Immunogen:

cell division cycle 25 homolog B(S. pombe)

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

60-70 kDa

Form:

liquid

Applications:

ELISA, WB, IHC, IP

Recommended dilution:

WB: 1:200-1:1000; IP: 1:200-1:1000; IHC: 1:20-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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