Background:

CTCFL, also named BORIS(for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region(ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

Clonality:

monoclonal

Classification:

Primary antibody

Alternative names:

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, dJ579F20.2, Zinc finger protein CTCF T

Specificity:

Human, Mouse

Host:

Mouse

Isotype:

IgG1

Immunogen:

CCCTC-binding factor(zinc finger protein)-like

Purification:

Protein A+G purification

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

75 kDa

Form:

liquid

Applications:

ELISA, WB, IF

Recommended dilution:

WB: 1:500-1:2000; IF: 1:10-1:100

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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