Background:

Dioxygenase that acts as a DNA demethylase(PubMed:18603530). Requires molecular oxygen, alpha-ketoglutarate and iron(PubMed:18603530). Specifically demethylates DNA methylated on the 6th position of adenine(N(6)-methyladenosine) DNA. N(6)-methyladenosine(m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing(By similarity). Also able to repair alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation, but with low activity(PubMed:18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites(PubMed:19959401). DNA lyase activity does not require alpha-ketoglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product(PubMed:19959401, PubMed:23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation(By similarity).

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

ABH, ABH1, alkB, ALKBH, ALKBH1, DNA lyase ABH1, hABH

Specificity:

Human, Mouse, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

alkB, alkylation repair homolog 1(E. coli)

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

44 kDa, observed 44 kDa

Form:

liquid

Applications:

ELISA, WB, IHC

Recommended dilution:

WB: 1:200-1:2000; IHC: 1:20-1:200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

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Email: info@sobekbio.com

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