Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin(a2M). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin(a2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin(a2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin(a2M) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Tumor immunity;Infection immunity;Hematology;

Species reactivity:

Horse

Alternative names:

α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Sample type:

serum, plasma, urine and other biological fluids

Detection range:

Sensitivity:

0.35 ng/mL

Standard:

50 ng/mL

Detection method:

Sandwich

Assay time:

4.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com