Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Beta(IL8Rb). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 8 Receptor Beta(IL8Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Beta(IL8Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 Receptor Beta(IL8Rb) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Tumor immunity;Infection immunity;Dermatology;

Species reactivity:

Pig

Alternative names:

IL-8RB;CD182; CDw128b; IL8R-B; IL8-RB; CMKAR2; CXCR2; IL8R2; C-X-C chemokine receptor type 2; GRO/MGSA receptor; High affinity interleukin-8 receptor B

Sample type:

Tissue homogenates, cell lysates and other biological fluids

Detection range:

0.79-50 ng/mL

Sensitivity:

0.27 ng/mL

Standard:

50 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com