Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenomedullin(ADM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adrenomedullin(ADM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenomedullin(ADM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenomedullin(ADM) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Endocrinology;Hormone metabolism;

Species reactivity:

Cattle

Alternative names:

AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide

Sample type:

serum, plasma, tissue homogenates and other biological fluids

Detection range:

Sensitivity:

5.5 pg/mL

Standard:

1000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com