Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholecystokinin A Receptor(CCKAR). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholecystokinin A Receptor(CCKAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholecystokinin A Receptor(CCKAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholecystokinin A Receptor(CCKAR) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Endocrinology;Hepatology;Hormone metabolism;

Species reactivity:

Rabbit

Alternative names:

CCK-A; CCK1; CCKRA; CCK1R; Cholecystokinin Type 1 Receptor

Sample type:

Tissue homogenates and other biological fluids.

Detection range:

Sensitivity:

0.61 ng/mL

Standard:

100 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com