Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Alpha(IL8Ra). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 8 Receptor Alpha(IL8Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Alpha(IL8Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 Receptor Alpha(IL8Ra) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;CD & Adhesion molecule;Infection immunity;

Species reactivity:

Rabbit

Alternative names:

IL-8RA;CD181; CDw128a; CXCR1; CKR1; CMKAR1; IL8R1; IL8RBA; IL8-RA; Chemokine C-X-C motif Receptor 1; High Affinity Interleukin-8 Receptor A

Sample type:

Serum, plasma, tissue homogenates and other biological fluids.

Detection range:

0.16-10 ng/mL

Sensitivity:

0.055 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com