Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype B(MUC5B). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mucin 5 Subtype B(MUC5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype B(MUC5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 5 Subtype B(MUC5B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Tumor immunity;Infection immunity;Pulmonology;Dermatology;

Species reactivity:

Mouse

Alternative names:

MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin

Sample type:

tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

0.239 ng/mL

Standard:

40 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com