Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-Thromboglobulin(bTG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-Thromboglobulin(bTG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-Thromboglobulin(bTG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Thromboglobulin(bTG) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Cytokine;Hematology;

Species reactivity:

Mouse

Alternative names:

CXCL7; PPBP; PBP; B-TG1; CTAP-III; CTAP3; CTAPIII; LA-PF4; LDGF; MDGF; NAP2; SCYB7; TC1; TC2; TGB1; THBGB1; Pro-Platelet Basic Protein; Chemokine C-X-C-Motif Ligand 7

Sample type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection range:

Sensitivity:

0.65 pg/mL

Standard:

100 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com