Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin(a1AT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin(a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin(a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin(a1AT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Enzyme & Kinase;Infection immunity;Hepatology;

Species reactivity:

Mouse

Alternative names:

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

Sample type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

13.7 ng/mL

Standard:

2000 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com