Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Receptor 1(CX3CR1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Receptor 1(CX3CR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X3-C-Motif Receptor 1(CX3CR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X3-C-Motif Receptor 1(CX3CR1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

CD & Adhesion molecule;

Species reactivity:

Mouse

Alternative names:

CCRL1; CMKBRL1; CMKDR1; GPR13; GPRV28; V28; Beta chemokine receptor-like 1; Fractalkine receptor; G-protein coupled receptor 13

Sample type:

tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

0.055 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com