Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 Receptor Alpha(IL5Ra). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 5 Receptor Alpha(IL5Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 5 Receptor Alpha(IL5Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 5 Receptor Alpha(IL5Ra) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;CD & Adhesion molecule;Infection immunity;

Species reactivity:

Mouse

Alternative names:

CDw125; CD125; IL5R-A; IL5-RA; HSIL5R3; IL5R; IL5R-A;IL-5RA

Sample type:

Serum, plasma and other biological fluids.

Detection range:

62.5-4000 pg/mL

Sensitivity:

28.5 pg/mL

Standard:

4000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com