Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein(Bax). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Associated X Protein(Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein(Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein(Bax) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Apoptosis;

Species reactivity:

Mouse

Alternative names:

BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4

Sample type:

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

26.3 pg/mL

Standard:

4000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com