Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Beta Peptide 1-42(Ab1-42). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Amyloid Beta Peptide 1-42(Ab1-42). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amyloid Beta Peptide 1-42(Ab1-42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Amyloid Beta Peptide 1-42(Ab1-42) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Neuro science;

Species reactivity:

Mouse

Alternative names:

Amyloid beta 42(ABeta 42);amyloid beta peptide 1-42;Aβ1-42,amyloid βpeptide 1-42

Sample type:

serum, plasma and other biological fluids

Detection range:

Sensitivity:

2.64 pg/mL

Standard:

500 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com