Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+/K+ Exchanging Alpha Polypeptide(ATP4a). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATPase, H+/K+ Exchanging Alpha Polypeptide(ATP4a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+/K+ Exchanging Alpha Polypeptide(ATP4a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+/K+ Exchanging Alpha Polypeptide(ATP4a) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;

Species reactivity:

Mouse

Alternative names:

Gastric H,K-ATPase Alpha Subunit; H(+)-K(+)-ATPase Alpha Subunit; Proton Pump; Potassium-transporting ATPase alpha chain 1

Sample type:

Tissue homogenates and other biological fluids.

Detection range:

Sensitivity:

0.055 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com