Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesoid X Receptor(FXR). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Farnesoid X Receptor(FXR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesoid X Receptor(FXR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Farnesoid X Receptor(FXR) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;

Species reactivity:

Mouse

Alternative names:

NR1H4; BAR; FXR; HRR1; RIP14; Nuclear Receptor Subfamily 1,Group H,Member 4; Bile acid receptor; Farnesol receptor HRR-1; Retinoid X receptor-interacting protein 14

Sample type:

Tissue homogenates and other biological fluids.

Detection range:

Sensitivity:

0.051 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com