Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5(HSPA5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 5(HSPA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 5(HSPA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 5(HSPA5) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Tumor immunity;Infection immunity;

Species reactivity:

Mouse

Alternative names:

MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78

Sample type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

0.057 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com