Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Damage Inducible Transcript 3(DDIT3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DNA Damage Inducible Transcript 3(DDIT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Damage Inducible Transcript 3(DDIT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Damage Inducible Transcript 3(DDIT3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;

Species reactivity:

Mouse

Alternative names:

CEBPZ; CHOP; CHOP10; GADD153; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible GADD153

Sample type:

tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

0.056 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com