Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement component 1, s subcomponent(C1S1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement component 1, s subcomponent(C1S1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement component 1, s subcomponent(C1S1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement component 1, s subcomponent(C1S1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Immunology

Species reactivity:

Mouse

Alternative names:

Basic proline rich peptide IB 1 ; C1 esterase ; C1S ; Complement C1s subcomponent ; Complement C1s subcomponent heavy chain ; Complement C1s subcomponent light chain ; Complement component 1 s subcomponent ; Complement component 1 subcomponent s ; FLJ44757

Sample type:

serum, plasma, tissue homogenates, and other biological fluids

Detection range:

Sensitivity:

6.65ng/mL

Standard:

1000 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com