Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 5(KCNJ5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 5(KCNJ5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Inwardly Rectifying Channel Subfamily J, Member 5(KCNJ5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Potassium Inwardly Rectifying Channel Subfamily J, Member 5(KCNJ5) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;

Species reactivity:

Rat

Alternative names:

Kir3.4; CIR; KATP1; GIRK4; G protein-activated inward rectifier potassium channel 4; Cardiac inward rectifier; Inward rectifier K(+) channel Kir3.4; Heart KATP channel

Sample type:

Tissue homogenates and other biological fluids.

Detection range:

Sensitivity:

0.055 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com