Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3(C3c). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C3(C3c). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C3(C3c), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C3(C3c) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

serum, plasma and other biological fluids

Species reactivity:

Rat

Alternative names:

ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein

Sample type:

serum, plasma, cell lysates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

1.37 ng/mL

Standard:

200 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com