Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 Receptor(IGF2R). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 Receptor(IGF2R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 Receptor(IGF2R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 2 Receptor(IGF2R) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Endocrinology;

Species reactivity:

Human

Alternative names:

CD222; IGF2-R; CIMPR; M6P-R; MPRI; Cation-Independent Mannose-6 Phosphate Receptor; M6P/IGF2 receptor; 300 kDa mannose 6-phosphate receptor

Sample type:

Serum, plasma and other biological fluids.

Detection range:

Sensitivity:

0.221 ng/mL

Standard:

40 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com