Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1,3-Glucuronyltransferase 1(b3GAT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-1,3-Glucuronyltransferase 1(b3GAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1,3-Glucuronyltransferase 1(b3GAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-1,3-Glucuronyltransferase 1(b3GAT1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

CD & Adhesion molecule;Metabolic pathway;Neuro science;Hepatology;

Species reactivity:

Human

Alternative names:

CD57; B3-GAT1; GLCATP; GlcAT-P; GlcUAT-P; HNK-1; LEU7; NK-1; Glucuronosyltransferase P; Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1

Sample type:

Tissue homogenates, cell lysates and other biological fluids.

Detection range:

Sensitivity:

0.053 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com