Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha(FLNa). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Filamin A Alpha(FLNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin A Alpha(FLNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin A Alpha(FLNa) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Developmental science;

Species reactivity:

Human

Alternative names:

ABP280; FLN-A; ABPX; FLN; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; Actin Binding Protein 280; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin

Sample type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

0.65 ng/mL

Standard:

120 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com