Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2B(H2B). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Histone H2B(H2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H2B(H2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H2B(H2B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Developmental science;

Species reactivity:

Human

Alternative names:

HIST1H2BA; testis; bA317E16.3; H2B; H2B histone family member U; H2B histone family member U testis specific; H2B histone family, member U, (testis specific); H2B testis; H2B1A_HUMAN; H2BFU; H2BT; HIST1H2BA; Histone 1, H2ba; Histone cluster 1 H2ba; Histone H2B; Histone H2B testis; Histone H2B type 1 A; Histone H2B type 1-A; Histone H2B type 1A; STBP; Testis specific histone H2B; Testis-specific histone H2B; TSH 2B; TSH2B; TSH2B.1;

Sample type:

tissue homogenates, cell lysates and other biological fluids

Detection range:

3.13-200 ng/mL

Sensitivity:

1.18 ng/mL

Standard:

200 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com