Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell, Immune Regulator 1(TCIRG1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T-Cell, Immune Regulator 1(TCIRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell, Immune Regulator 1(TCIRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell, Immune Regulator 1(TCIRG1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Infection immunity;

Species reactivity:

Human

Alternative names:

Stv1; Vph1; ATP6N1C; ATP6V0A3; Atp6i; OC-116kDa; OC116; OPTB1; TIRC7; a3; V-Type Proton ATPase 116 kDa Subunit A Isoform 3; Osteoclastic proton pump 116 kDa subunit

Sample type:

Serum and other biological fluids.

Detection range:

Sensitivity:

6 pg/mL

Standard:

1000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com